dcas9 vp64 (Addgene inc)
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dcas9 vp64
Dcas9 Vp64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+dcas9+vp64+plasmid/pcDNA-dCas9-VP64+(Plasmid+%2347107)/pmc12706070-625-7-12
Average 93 stars, based on 68 article reviews
Dcas9 Vp64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+dcas9+vp64+plasmid/pcDNA-dCas9-VP64+(Plasmid+%2347107)/pmc12706070-625-7-12
Average 93 stars, based on 68 article reviews
dcas9 vp64 - by Bioz Stars,
2026-10
93/100 stars
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Sequencing:Article Title: Efficient activation of hundreds of LTR12C elements reveals cis -regulatory function determined by distinct epigenetic mechanisms Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9-SunTag-p300. .. The VP64 sequence was amplified from Article Title: Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators Article Snippet: To generate Fluc reporter vectors, the UFGT and CBF4 promoters were amplified from the pLB vectors and ligated into the pCAMBIA1302-Fluc vector, respectively, via homologous recombination (HR) using the ClonExpress II One Step Cloning Kit (Vazyme, China). .. To develop the dCas9-VP64 expression vector, the dCas9-VP64 coding sequence was amplified from the Article Title: Efficient activation of hundreds of LTR12C elements reveals cis-regulatory function determined by distinct epigenetic mechanisms. Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9SunTag-p300. .. The VP64 sequence was amplified from Amplification:Article Title: Efficient activation of hundreds of LTR12C elements reveals cis -regulatory function determined by distinct epigenetic mechanisms Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9-SunTag-p300. .. The VP64 sequence was amplified from Article Title: Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators Article Snippet: To generate Fluc reporter vectors, the UFGT and CBF4 promoters were amplified from the pLB vectors and ligated into the pCAMBIA1302-Fluc vector, respectively, via homologous recombination (HR) using the ClonExpress II One Step Cloning Kit (Vazyme, China). .. To develop the dCas9-VP64 expression vector, the dCas9-VP64 coding sequence was amplified from the Article Title: Efficient activation of hundreds of LTR12C elements reveals cis-regulatory function determined by distinct epigenetic mechanisms. Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9SunTag-p300. .. The VP64 sequence was amplified from Plasmid Preparation:Article Title: Efficient activation of hundreds of LTR12C elements reveals cis -regulatory function determined by distinct epigenetic mechanisms Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9-SunTag-p300. .. The VP64 sequence was amplified from Article Title: Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators Article Snippet: To generate Fluc reporter vectors, the UFGT and CBF4 promoters were amplified from the pLB vectors and ligated into the pCAMBIA1302-Fluc vector, respectively, via homologous recombination (HR) using the ClonExpress II One Step Cloning Kit (Vazyme, China). .. To develop the dCas9-VP64 expression vector, the dCas9-VP64 coding sequence was amplified from the Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Article Snippet: When the cell density reached 70–80%, polyethylenimine (PEI, Sigma, St. Louis, MO, USA) was used for transfection. .. Cells were co-transfected with 2 μg of the recombinant plasmid and 2 μg of Article Title: Optical Control of Base Editing and Transcription through Light‐Activated Guide RNA Article Snippet: We developed a method to control gene activation and base editing in mammalian cells and zebrafish embryos via photochemically activated, caged guide RNAs.. Four evenly distributed, caged nucleobases are installed in the 5’-protospacer region of the gRNA to block interaction between the dCas9:gRNA complex and the dsDNA target.. Upon light activation, gRNA:dsDNA hybridization is restored and catalytically dead Cas9-based CRISPR activation or base editing is achieved. Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway Article Snippet: When the cell density reached 70–80%, polyethylenimine (PEI, Sigma, St. Louis, MO, USA) was used for transfection. .. Cells were co-transfected with 2 μg of the recombinant plasmid and 2 μg of Article Title: Efficient activation of hundreds of LTR12C elements reveals cis-regulatory function determined by distinct epigenetic mechanisms. Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9SunTag-p300. .. The VP64 sequence was amplified from Polymerase Chain Reaction:Article Title: Efficient activation of hundreds of LTR12C elements reveals cis -regulatory function determined by distinct epigenetic mechanisms Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9-SunTag-p300. .. The VP64 sequence was amplified from Article Title: Efficient activation of hundreds of LTR12C elements reveals cis-regulatory function determined by distinct epigenetic mechanisms. Article Snippet: pPlatTET-gRNA2 (Addgene #82559) plasmid served as a source vector to create dCas9-SunTag-VP64 and dCas9SunTag-p300. .. The VP64 sequence was amplified from Expressing:Article Title: Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators Article Snippet: To generate Fluc reporter vectors, the UFGT and CBF4 promoters were amplified from the pLB vectors and ligated into the pCAMBIA1302-Fluc vector, respectively, via homologous recombination (HR) using the ClonExpress II One Step Cloning Kit (Vazyme, China). .. To develop the dCas9-VP64 expression vector, the dCas9-VP64 coding sequence was amplified from the Recombinant:Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Article Snippet: When the cell density reached 70–80%, polyethylenimine (PEI, Sigma, St. Louis, MO, USA) was used for transfection. .. Cells were co-transfected with 2 μg of the recombinant plasmid and 2 μg of Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway Article Snippet: When the cell density reached 70–80%, polyethylenimine (PEI, Sigma, St. Louis, MO, USA) was used for transfection. .. Cells were co-transfected with 2 μg of the recombinant plasmid and 2 μg of Incubation:Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Article Snippet: When the cell density reached 70–80%, polyethylenimine (PEI, Sigma, St. Louis, MO, USA) was used for transfection. .. Cells were co-transfected with 2 μg of the recombinant plasmid and 2 μg of Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway Article Snippet: When the cell density reached 70–80%, polyethylenimine (PEI, Sigma, St. Louis, MO, USA) was used for transfection. .. Cells were co-transfected with 2 μg of the recombinant plasmid and 2 μg of |
![The dCas9-SunTag-based CRISPRa systems induce LTR12C expression from proximal promoter regions. ( A ) Percentage of upregulated LTR families in human cell lines following 5-aza-CdR treatment. The four cell lines were treated with PBS (control) or 300 nM 5-aza-CdR for 24 h and harvested at 5 days after the treatment. The upregulated LTR copies [fold change (FC) >2 by comparison with PBS-treated human cell lines] are categorized into each LTR family. The top 10 most highly abundant LTR families are highlighted with distinct colors. ( B ) Schematics of dCas9 constructs and fusion proteins. The constructs contain dCas9, SunTags separated by 22-amino-acid linkers (Multi SunTag), 2A self-cleaving peptide (2A), single-chain variable fragment (ScFv), green fluorescence protein (GFP), <t>VP64/p300</t> and gRNA. The fusion proteins with single gRNA are inferred to transactivate multiple copies of LTR12C. ( C ) A sequence alignment of LTR12C elements deposited in the RepeatMasker database. Each row represents one LTR12C element. Heatmap indicates percent identity (0 to 90%); blank indicates gaps. Arrowhead indicates gRNA positions. Promoter and enhancer region is defined as 400 bp upstream region from TSS. ( D ) Relative expression of LTR12C based on reverse transcription qPCR (RT-qPCR) after transfection of the dCas9-SunTag-VP64 construct with represented gRNAs in HEK293T cells. Error bars represent standard error of the mean (SEM) from three independent biological replicates.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7136/pmc11317136/pmc11317136__gkae498fig1.jpg)
